| First Author | Washio K | Year | 2015 |
| Journal | Genes Cells | Volume | 20 |
| Issue | 6 | Pages | 451-63 |
| PubMed ID | 25818708 | Mgi Jnum | J:230340 |
| Mgi Id | MGI:5758793 | Doi | 10.1111/gtc.12238 |
| Citation | Washio K, et al. (2015) Dendritic cell SIRPalpha regulates homeostasis of dendritic cells in lymphoid organs. Genes Cells 20(6):451-63 |
| abstractText | Signal regulatory protein alpha (SIRPalpha), an immunoglobulin superfamily protein that is expressed predominantly in myeloid lineage cells such as dendritic cells (DCs) or macrophages, mediates cell-cell signaling. In the immune system, SIRPalpha is thought to be important for homeostasis of DCs, but it remains unclear whether SIRPalpha intrinsic to DCs is indeed indispensable for such functional role. Thus, we here generated the mice, in which SIRPalpha was specifically ablated in CD11c(+) DCs (Sirpa(Delta) (DC) ). Sirpa(Delta) (DC) mice manifested a marked reduction of CD4(+) CD8alpha(-) conventional DCs (cDCs) in the secondary lymphoid organs, as well as of Langerhans cells in the epidermis. Such reduction of cDCs in Sirpa(Delta) (DC) mice was comparable to that apparent with the mice, in which SIRPalpha was systemically ablated. Expression of SIRPalpha in DCs was well correlated with that of either endothelial cell-selective adhesion molecule (ESAM) or Epstein-Barr virus-induced molecule 2 (EBI2), both of which were also implicated in the regulation of DC homeostasis. Indeed, ESAM(+) or EBI2(+) cDCs were markedly reduced in the spleen of Sirpa(Delta) (DC) mice. Thus, our results suggest that SIRPalpha intrinsic to CD11c(+) DCs is essential for homeostasis of cDCs in the secondary lymphoid organs and skin. |
