| First Author | Endersby R | Year | 2008 |
| Journal | Blood | Volume | 111 |
| Issue | 4 | Pages | 1946-50 |
| PubMed ID | 18063753 | Mgi Jnum | J:131310 |
| Mgi Id | MGI:3773482 | Doi | 10.1182/blood-2007-04-085746 |
| Citation | Endersby R, et al. (2008) Hls5 regulated erythroid differentiation by modulating GATA-1 activity. Blood 111(4):1946-50 |
| abstractText | Hemopoietic lineage switch (Hls) 5 and 7 were originally isolated as genes up-regulated during an erythroid-to-myeloid lineage switch. We have shown previously that Hls7/Mlf1 imposes a monoblastoid phenotype on erythroleukemic cells. Here we show that Hls5 impedes erythroid maturation by restricting proliferation and inhibiting hemoglobin synthesis; however, Hls5 does not influence the morphology of erythroid cells. Under the influence of GATA-1, Hls5 relocates from cytoplasmic granules to the nucleus where it associates with both FOG-1 and GATA-1. In the nucleus, Hls5 is able to suppress GATA-1-mediated transactivation and reduce GATA-1 binding to DNA. We conclude that Hls5 and Hls7/Mlf1 act cooperatively to induce biochemical and phenotypic changes associated with erythroid/myeloid lineage switching. |
