| Primary Identifier | MGI:3713747 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sirt1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neomycin cassette was inserted just upstream of exon 4 and a third loxP site was inserted just downstream. The floxed mutation does not affect the protein expression of the targeted gene in MEF's or mammary tissue from homozygotes. Exon 4 (encoding a conserved Sir2 motif) was then removed by cre-mediated recombination to create a null allele. Absence of protein product was confirmed by western blot analysis. |
