| First Author | Riordan JR | Year | 1985 |
| Journal | Nature | Volume | 316 |
| Issue | 6031 | Pages | 817-9 |
| PubMed ID | 2863759 | Mgi Jnum | J:32391 |
| Mgi Id | MGI:79888 | Doi | 10.1038/316817a0 |
| Citation | Riordan JR, et al. (1985) Amplification of P-glycoprotein genes in multidrug-resistant mammalian cell lines. Nature 316(6031):817-9 |
| abstractText | The multidrug-resistance phenotype expressed in mammalian cell lines is complex. Cells selected with a single agent can acquire cross-resistance to a remarkably wide range of compounds which have no obvious structural or functional similarities. The basis for cross-resistance seems to be a decreased net cellular accumulation of the drug involved, and has been attributed to alterations in the plasma membrane. An over-expressed plasma membrane glycoprotein of relative molecular mass (Mr) 170,000 (P-glycoprotein) is consistently found in different multidrug-resistant human and animal cell lines, and in transplantable tumours. Consequently, it has been postulated that P-glycoprotein directly or indirectly mediates multidrug resistance. Here we report the cloning of a complementary DNA encoding P-glycoprotein. Southern blot analysis of hamster, mouse and human DNA using this cDNA as a probe showed that P-glycoprotein is conserved and is probably encoded by a gene family, and that members of this putative family are amplified in multidrug-resistant cells. |
