| First Author | Wydner KS | Year | 1997 |
| Journal | Genomics | Volume | 40 |
| Issue | 2 | Pages | 342-5 |
| PubMed ID | 9119402 | Mgi Jnum | J:39119 |
| Mgi Id | MGI:86500 | Doi | 10.1006/geno.1996.4574 |
| Citation | Wydner KS, et al. (1997) An intron capture strategy used to identify and map a lysyl oxidase-like gene on chromosome 9 in the mouse. Genomics 40(2):342-5 |
| abstractText | An intron capture strategy involving use of polymerase chain reaction was used to identify and map the mouse homologue of a human lysyl oxidase-like gene (LOXL). Oligonucleotides complementary to conserved domains within exons 4 and 5 of the human lysyl oxidase-like gene were used to amplify the corresponding segment from mouse genomic DNA. Sequencing of the resulting mouse DNA fragment of approximately 1 kb revealed that the exon sequences at the ends of the amplified fragment are highly homologous (90% nucleotide identity) to exons 4 and 5 of the human lysyl oxidase-like gene. An AluI restriction site polymorphism within intron 4 was used to map the mouse lysyl oxidase-like gene (Loxl) to mouse Chromosome 9 in a region that shares linkage conservation with human chromosome 15q24, to which the LOXL was recently mapped. (C) 1997 Academic Press. |
