| First Author | Lee CG | Year | 1994 |
| Journal | J Immunol | Volume | 152 |
| Issue | 12 | Pages | 5758-67 |
| PubMed ID | 8207206 | Mgi Jnum | J:18633 |
| Mgi Id | MGI:66892 | Doi | 10.4049/jimmunol.152.12.5758 |
| Citation | Lee CG, et al. (1994) Molecular cloning and characterization of a murine LPS-inducible cDNA. J Immunol 152(12):5758-67 |
| abstractText | Murine macrophages respond to endotoxins by inducing a vast array of genes that play a major role in the host's response to infection and tumor growth. We have isolated and characterized a 1.8-kb cDNA, designated IRG2, from a cDNA library prepared from RNA isolated from the murine cell line, RAW 264.7, after bacterial LPS stimulation. The cDNA encodes a protein of 47 kDa that is the murine homologue of a small family of proteins described from IFN-induced human cells. The IRG2 message does not appear until 3 h after LPS exposure and its induction is dependent on new protein synthesis. IRG2 induction by LPS is slightly inhibited by the anti-inflammatory steroid, dexamethasone. Increasing cytosolic cAMP with either forskolin, dibutyryl cAMP, or 8-(4-chlorophenylthio)-cAMP caused marked inhibition of the LPS induction of IRG2. In contrast, activation of PKC with phorbol ester potentiated the LPS response. Removing extracellular Ca2+ with EGTA inhibited IRG2 induction; increasing intracellular calcium with the calcium ionophore A23187 led to enhanced levels of the IRG2 transcript. These data suggest that the induction of IRG2 occurs via a PKC pathway. |
