| First Author | Orchard RC | Year | 2016 |
| Journal | Science | Volume | 353 |
| Issue | 6302 | Pages | 933-6 |
| PubMed ID | 27540007 | Mgi Jnum | J:236914 |
| Mgi Id | MGI:5810217 | Doi | 10.1126/science.aaf1220 |
| Citation | Orchard RC, et al. (2016) Discovery of a proteinaceous cellular receptor for a norovirus. Science 353(6302):933-6 |
| abstractText | Noroviruses (NoVs) are a leading cause of gastroenteritis globally, yet the host factors required for NoV infection are poorly understood. We identified host molecules that are essential for murine NoV (MNoV)-induced cell death, including CD300lf as a proteinaceous receptor. We found that CD300lf is essential for MNoV binding and replication in cell lines and primary cells. Additionally, Cd300lf(-/-) mice are resistant to MNoV infection. Expression of CD300lf in human cells breaks the species barrier that would otherwise restrict MNoV replication. The crystal structure of the CD300lf ectodomain reveals a potential ligand-binding cleft composed of residues that are critical for MNoV infection. Therefore, the presence of a proteinaceous receptor is the primary determinant of MNoV species tropism, whereas other components of cellular machinery required for NoV replication are conserved between humans and mice. |
