Primary Identifier | MGI:6357174 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Pcgf6 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
description | ES Cell line was either R1 or M1. |
molecularNote | LoxP sites and an FRT-flanked neomycin selection cassette were inserted to flank exons 2 and 3. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination in the germline resulted in the deletion of exons 2 and 3 and the neomycin selection cassette. Northern analysis confirmed the absence of mRNA expression in adult homozygous mutant kidneys. |