| Primary Identifier | MGI:2449915 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Hspg2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3 and flanking regions were deleted and replaced with a PGK-neo cassette via homologous recombination. The targeted mutation removes the heparan sulfate attachment while the core protein remains intact. RT-PC analysis of RNA from homozygous mutant embryos confirmed the exon 3 deletion. Fibroblasts derived from homozygous mutant cell culture were treated with heparitinase and chondroitinase, run on an SDS-PAGE gel, and immunoblotted to confirm the mutant protein retained the core protein and lacked heparan sulfate side chains. |
