| Primary Identifier | MGI:5576261 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, No functional change | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The Merkel cell polyomavirus (MCPyV) early region, isolated from MCC tumor specimen MCCw168 (MCPyV168; GenBank: KC426954.1), was PCR amplified and cloned into the pBigT vector between XhoI and NotI restriction sites. A 6kb fragment containing the neomycin resistance gene and triple SV40 polyadenylation transcription terminators flanked by two loxP sites (loxP-stop-loxP, or LSL), MCPyV168, and bovine growth hormone polyadenylation terminator were cut from the pBigT vector and cloned into the pROSA26PA vector between PacI and AscI restriction sites. |
