| First Author | Hongo D | Year | 2021 |
| Journal | Front Immunol | Volume | 12 |
| Pages | 746469 | PubMed ID | 34777358 |
| Mgi Jnum | J:314699 | Mgi Id | MGI:6826095 |
| Doi | 10.3389/fimmu.2021.746469 | Citation | Hongo D, et al. (2021) Identification of Two Subsets of Murine DC1 Dendritic Cells That Differ by Surface Phenotype, Gene Expression, and Function. Front Immunol 12:746469 |
| abstractText | Classical dendritic cells (cDCs) in mice have been divided into 2 major subsets based on the expression of nuclear transcription factors: a CD8(+)Irf8(+)Batf3 dependent (DC1) subset, and a CD8(-)Irf4(+) (DC2) subset. We found that the CD8(+)DC1 subset can be further divided into CD8(+)DC1a and CD8(+)DC1b subsets by differences in surface receptors, gene expression, and function. Whereas all 3 DC subsets can act alone to induce potent Th1 cytokine responses to class I and II MHC restricted peptides derived from ovalbumin (OVA) by OT-I and OT-II transgenic T cells, only the DC1b subset could effectively present glycolipid antigens to natural killer T (NKT) cells. Vaccination with OVA protein pulsed DC1b and DC2 cells were more effective in reducing the growth of the B16-OVA melanoma as compared to pulsed DC1a cells in wild type mice. In conclusion, the Batf3-/- dependent DC1 cells can be further divided into two subsets with different immune functional profiles in vitro and in vivo. |
