| First Author | Cheng G | Year | 1995 |
| Journal | Science | Volume | 267 |
| Issue | 5203 | Pages | 1494-8 |
| PubMed ID | 7533327 | Mgi Jnum | J:34086 |
| Mgi Id | MGI:81560 | Doi | 10.1126/science.7533327 |
| Citation | Cheng G, et al. (1995) Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 267(5203):1494-8 |
| abstractText | CD40 is a receptor on the surface of B lymphocytes, the activation of which leads to B cell survival, growth, and differentiation. A yeast two-hybrid screen identified a gene, CRAF1, encoding a protein that interacts directly with the CD40 cytoplasmic tail through a region of similarity to the tumor necrosis factor-alpha (TNF-alpha) receptor-associated factors. Overexpression of a truncated CRAF1 gene inhibited CD40-mediated up-regulation of CD23. A region of CRAF1 was similar to the TNF-alpha receptor-associated factors TRAF1 and TRAF2 and so defined a shared TRAF-C domain that was necessary and sufficient for CD40 binding and homodimerization. The CRAF1 sequence also predicted a long amphipathic helix, a pattern of five zinc fingers, and a zinc ring finger. It is likely that other members of the TNF receptor superfamily use CRAF-related proteins in their signal transduction processes. |
