| First Author | Yi JH | Year | 2014 |
| Journal | Exp Neurol | Volume | 255 |
| Pages | 12-8 | PubMed ID | 24530640 |
| Mgi Jnum | J:212036 | Mgi Id | MGI:5577230 |
| Doi | 10.1016/j.expneurol.2014.02.007 | Citation | Yi JH, et al. (2014) Receptor protein tyrosine phosphatase sigma binds to neurons in the adult mouse brain. Exp Neurol 255:12-8 |
| abstractText | The role of type IIA receptor protein tyrosine phosphatases (RPTPs), which includes LAR, RPTPsigma and RPTPdelta, in the nervous system is becoming increasingly recognized. Evidence supports a significant role for these RPTPs during the development of the nervous system as well as after injury, and mutations in RPTPs are associated with human disease. However, a major open question is the nature of the ligands that interact with type IIA RPTPs in the adult brain. Candidates include several different proteins as well as the glycosaminoglycan chains of proteoglycans. In order to investigate this problem, we used a receptor affinity probe assay with RPTPsigma-AP fusion proteins on sections of adult mouse brain and to cultured neurons. Our results demonstrate that the major binding sites for RPTPsigma in adult mouse brain are on neurons and are not proteoglycan GAG chains, as RPTPsigma binding overlaps with the neuronal marker NeuN and was not significantly altered by treatments which eliminate chondroitin sulfate, heparan sulfate, or both. We also demonstrate no overlap of binding of RPTPsigma with perineuronal nets, and a unique modulation of RPTPsigma binding to brain by divalent cations. Our data therefore point to neuronal proteins, rather than CSPGs, as being the ligands for RPTPsigma in the adult, uninjured brain. |
