Primary Identifier | MGI:7377719 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready | Gene | Pgp |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
description | C57BL/6 ES cells contain flp recombinase. |
molecularNote | CRISPR/Cas9 technology flanked exons 1 and 2 with loxP sites and inserted an FRT-flanked neomycin selection cassette after exon 2. The selection cassette was removed via flp-mediated recombination in ES cells expressing flp recombinase. |