Primary Identifier | MGI:6791388 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase | Gene | Nkx2-5 |
Transmission | Germline | Strain of Origin | Not Specified |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | Using homologous recombination in ES cells an internal ribosome entry site (IRES) linked to the gene encoding a nuclear-localizing recombinase cre fused to ERT2 and a hygromycin resistance gene cassette (pgk-HYGROpA) flanked by yeast flp recombinase target (frt) sites was inserted into the 3' UTR of the endogenous locus. pgk-HYGRO-pA cassette was removed by crossing to Flp recombinase gene expressing mice. |