| First Author | LeGouy E | Year | 1998 |
| Journal | Dev Dyn | Volume | 212 |
| Issue | 1 | Pages | 38-48 |
| PubMed ID | 9603422 | Mgi Jnum | J:47287 |
| Mgi Id | MGI:1203262 | Doi | 10.1002/(SICI)1097-0177(199805)212:1<38::AID-AJA4>3.0.CO;2-3 |
| Citation | LeGouy E, et al. (1998) Differential preimplantation regulation of two mouse homologues of the yeast SWI2 protein. Dev Dyn 212(1):38-48 |
| abstractText | Epigenetic regulation of gene expression through modification of chromatin organization is an important mechanism in the development of eucaryotic organisms. We investigated the developmentally regulated expression of the mouse mBRG1 and mbrm genes, which are homologous to the yeast SWI2 gene. Both proteins are involved in chromatin remodeling as components of the mammalian SWI/SNF complex. The analysis was performed at a time in mouse development when the formation of a functional zygotic nucleus is closely linked to extensive chromatin modifications. Reverse transcription-polymerase chain reaction (RT-PCR) analysis in mature oocytes and through the first cleavage stages showed that both genes were highly expressed as maternal products but that they subsequently exhibited considerable differences in their level of expression when the transition to zygotic transcription occurred. Immunodetection of the two proteins with specific antibodies paralleled the RT-PCR analysis. The mBRG1 protein was present throughout preimplantation development, whereas zygotic mbrm was clearly detectable only when differentiation first occurs at the blastocyst stage. At this stage, mbrm was restricted to the inner cell mass. Cell type-specific expression of mbrm was also observed after in vitro differentiation of embryonic stem cells. These results indicate that the two murine homologues of SWI2 have substantially different roles in chromatin organization during the onset of embryonic development. |
