| Primary Identifier | MGI:2386545 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Nes-cre)1Wme |
| Strain of Origin | (C57BL/6 x CBA)F2 | Is Recombinase | true |
| Is Wild Type | false |
| description | Mice carrying this "balancer 1" transgene show less variability and instability in their generation of mosaics than do transgenic mice carrying the "balancer 2" transgene. |
| molecularNote | The transgenic construct contained a nestin promoter followed by cre recombinase sequence adjoined to a nuclear localization signal. Intron 2 of the nestin gene was included upstream of a polyadenylation signal. While the endogenous nestin promoter is CNS-specific, Southern blot analysis showed the ubiquitous expression of cre in transgenic mice. Variable mosaicism/partial deletion of loxP-flanked alleles occurs before embryonic day 10.5, and it is detectable in all adult organs examined including germ line cells. |
