| First Author | Ushio H | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 323 |
| Issue | 2 | Pages | 491-8 |
| PubMed ID | 15369778 | Mgi Jnum | J:92479 |
| Mgi Id | MGI:3052891 | Doi | 10.1016/j.bbrc.2004.08.108 |
| Citation | Ushio H, et al. (2004) MD-2 is required for the full responsiveness of mast cells to LPS but not to PGN. Biochem Biophys Res Commun 323(2):491-8 |
| abstractText | To address the role played by MD-2 in mast cell recognition of LPS, we examined bone marrow-derived mast cells (BMMCs) from MD-2 gene-targeted mice. BMMCs from MD-2-/- mice showed impaired cytokine production (TNF-alpha, IL-6, IL-13, and IL-1beta) in response to LPS from Escherichia coli, but not to peptidoglycan (PGN) from Staphylococcus aureus. In a mast cell-dependent acute septic model, MD-2 deficiency of mast cell resulted in significantly higher mortality due to defective neutrophil recruitment and the production of cytokines in the peritoneal cavity, which was similar to mice with TLR4-deficient mast cells. The TLR2-dependent activation of skin mast cells by PGN was not altered by the absence of MD-2 in vivo. Collectively, MD-2 is essential for the recognition of LPS by TLR4 but not for that of PGN by TLR2 of mast cells. |
