| First Author | Weaver C | Year | 2003 |
| Journal | Development | Volume | 130 |
| Issue | 22 | Pages | 5425-36 |
| PubMed ID | 14507779 | Mgi Jnum | J:85843 |
| Mgi Id | MGI:2677120 | Doi | 10.1242/dev.00737 |
| Citation | Weaver C, et al. (2003) GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs. Development 130(22):5425-36 |
| abstractText | In Xenopus, axis development is initiated by dorsally elevated levels of cytoplasmic beta-catenin, an intracellular factor regulated by GSK3 kinase activity. Upon fertilization, factors that increase beta-catenin stability are translocated to the prospective dorsal side of the embryo in a microtubule-dependent process. However, neither the identity of these factors nor the mechanism of their movement is understood. Here, we show that the GSK3 inhibitory protein GBP/Frat binds kinesin light chain (KLC), a component of the microtubule motor kinesin. Upon egg activation, GBP-GFP and KLC-GFP form particles and exhibit directed translocation. KLC, through a previously uncharacterized conserved domain, binds a region of GBP that is required for GBP translocation and for GSK3 binding, and competes with GSK3 for GBP. We propose a model in which conventional kinesin transports a GBP-containing complex to the future dorsal side, where GBP dissociates and contributes to the local stabilization of beta-catenin by binding and inhibiting GSK3. |
