| First Author | Thirion S | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 201 |
| Issue | 2 | Pages | 493-9 |
| PubMed ID | 8002978 | Mgi Jnum | J:18654 |
| Mgi Id | MGI:66910 | Doi | 10.1006/bbrc.1994.1729 |
| Citation | Thirion S, et al. (1994) Mouse macrophage derived monocyte chemotactic protein-3: cDNA cloning and identification as MARC/FIC. Biochem Biophys Res Commun 201(2):493-9 |
| abstractText | When the mouse macrophage cell line WEHI-3 is triggered with LPS it produces proteases and secondary cytokines including interleukin-6 and chemokines. In an attempt to isolate the mouse homologue of the human monocyte chemotactic protein-3 (MCP-3), a cDNA library from LPS-stimulated WEHI-3 cells was screened with the full-size human MCP-3 cDNA. The longest cDNA out of several positive clones was sequenced and encoded a protein of 97 residues. Except for a third codon letter mismatch it was identical to the mouse MARC cDNA and encoded the MARC protein. The murine Fic cDNA, which encodes a Marc-mutant protein with an arginine substitution for alanine, was not identified in the other sequenced homologous isolates. Similar to the human system, in which MCP-3 is most related to MCP-1, MURINE MCP-3 was found to be more homologous to mouse MCP-1/JE than to other murine C-C chemokines. We therefore postulate that MARC/FIC is the mouse MCP-3. |
