| First Author | Suh BC | Year | 1999 |
| Journal | Br J Pharmacol | Volume | 126 |
| Issue | 2 | Pages | 399-406 |
| PubMed ID | 10077231 | Mgi Jnum | J:53661 |
| Mgi Id | MGI:1333261 | Doi | 10.1038/sj.bjp.0702248 |
| Citation | Suh BC, et al. (1999) Pharmacological characterization of beta2-adrenoceptor in PGT-beta mouse pineal gland tumour cells. Br J Pharmacol 126(2):399-406 |
| abstractText | 1. The adrenoceptor in a mouse pineal gland tumour cell line (PGT-beta) was identified and characterized using pharmacological and physiological approaches. 2. Adrenaline and noradrenaline, adrenoceptor agonists, stimulated cyclic AMP generation in a concentration-dependent manner, but had no effect on inositol 1,4,5-trisphosphate production. Adrenaline was a more potent activator of cyclic AMP generation than noradrenaline, with half maximal-effective concentrations (EC50) seen at 175+/-22 nM and 18+/-2 microM for adrenaline and noradrenaline, respectively. 3. The addition of forskolin synergistically stimulated the adrenaline-mediated cyclic AMP generation in a concentration-dependent manner. 4. The pA2 value for the specific beta2-adrenoceptor antagonist ICI-118,551 (8.7+/-0.4) as an antagonist of the adrenaline-stimulated cyclic AMP generation were 3 units higher than the value for the betaI-adrenoceptor antagonist atenolol (5.6+/-0.3). 5. Treatment of the cells with adrenaline and forskolin evoked a 3 fold increase in the activity of serotonin N-acetyltransferase with the peak occurring 6 h after stimulation. 6. These results suggest the presence of beta2-adrenoceptors in mouse pineal cells and a functional relationship between the adenylyl cyclase system and the regulation of N-acetyltransferase expression. |
