| First Author | Gilly M | Year | 1992 |
| Journal | J Immunol | Volume | 148 |
| Issue | 10 | Pages | 3275-81 |
| PubMed ID | 1578148 | Mgi Jnum | J:17717 |
| Mgi Id | MGI:65748 | Doi | 10.4049/jimmunol.148.10.3275 |
| Citation | Gilly M, et al. (1992) The IRG-47 gene is IFN-gamma induced in B cells and encodes a protein with GTP-binding motifs. J Immunol 148(10):3275-81 |
| abstractText | The murine pre-B cell line 70Z/3 has been studied extensively as a model system for B cell differentiation induced by cytokines and mitogens. IFN-gamma treatment of 70Z/3 pre-B cells activates Ig kappa L chain gene expression, resulting in the appearance of surface IgM characteristic of B cells. We have isolated and characterized a new cDNA clone, called IRG-47, whose mRNA is transiently elevated up to 30-fold at 4 to 6 h after IFN-gamma induction of 70Z/3 pre-B cells. The IFN-gamma induction of IRG-47 mRNA occurs in all pre-B and in most B lineage cell lines examined but is only induced in three cell lines tested from other lineages. Activation of IRG-47 gene transcription appears to account for the rapid increase in IRG-47 mRNA after induction. Transcription of the IRG-47 gene is down-regulated 10 h postinduction at a time when the level of IRG-47 mRNA is also declining. The complete sequence of the IRG-47 cDNA clone is predicted to encode a 47-KDa protein which, although unique in its sequence, bears resemblance to a number of nucleotide-binding proteins. The IRG-47 coding sequence contains all three highly conserved peptide motifs in the appropriate spacing characteristic of guanine nucleotide-binding proteins. |
