| First Author | Jin G | Year | 2003 |
| Journal | Cancer Sci | Volume | 94 |
| Issue | 9 | Pages | 791-5 |
| PubMed ID | 12967477 | Mgi Jnum | J:86092 |
| Mgi Id | MGI:2678667 | Doi | 10.1111/j.1349-7006.2003.tb01520.x |
| Citation | Jin G, et al. (2003) Svi3: A provirus common integration site in c-myc in SL/Kh pre-B lymphomas. Cancer Sci 94(9):791-5 |
| abstractText | Spontaneous pre-B lymphomas in SL/Kh mice occur by somatic acquisition of a provirus genome of endogenous murine leukemia virus (MuLV). Inverse PCR amplification and sequence analyses of a provirus and its host flanking fragment revealed a proviral insertion into c-myc in 3 out of 60 SL/Kh pre-B lymphomas, named Svi3 lymphomas (SL/Kh virus integration site-3). Southern blot analysis revealed that two lymphomas had clonal integration in c-myc exon 1 and the other, in the promoter region. In 2 out of 3 Svi3 lymphomas, a fusion transcript of provirus 3' long terminal repeat and c-myc and a normal full-length c-myc transcript were obtained, but in one Svi3 lymphoma, only the normal transcript was obtained. All three Svi3 lymphomas had increased c-myc expression, producing normal 67-kDa c-Myc protein. Svi3 lymphomas had more mature phenotypes in the steps of early B-cell differentiation than Svi1 lymphomas, in which c-myc expression was indirectly up-regulated by provirus integration into Stat5a. |
