| First Author | Nakayama T | Year | 1999 |
| Journal | FEBS Lett | Volume | 453 |
| Issue | 1-2 | Pages | 77-80 |
| PubMed ID | 10403379 | Mgi Jnum | J:224747 |
| Mgi Id | MGI:5688850 | Doi | 10.1016/s0014-5793(99)00700-0 |
| Citation | Nakayama T, et al. (1999) Ca2(+)-dependent interaction of N-copine, a member of the two C2 domain protein family, with OS-9, the product of a gene frequently amplified in osteosarcoma. FEBS Lett 453(1-2):77-80 |
| abstractText | N-copine is a novel two C2 domain protein that shows Ca2(+)-dependent phospholipid binding and membrane association. By using yeast two-hybrid assays, we identified OS-9 as a protein capable of interacting with N-copine. We further revealed that the second C2 domain of N-copine bound with the carboxy-terminal region of OS-9. Their interaction in vivo was also confirmed by co-immunoprecipitation from 293E cells co-expressing transfected N-copine and OS-9. In vitro binding assays showed that this interaction was Ca2(+)-dependent. By Northern blot analysis, N-copine and OS-9 were co-expressed in the same regions of human brain. These results reveal that OS-9 is a potential target of N-copine. |
