| First Author | Tahirov TH | Year | 2002 |
| Journal | Cell | Volume | 108 |
| Issue | 1 | Pages | 57-70 |
| PubMed ID | 11792321 | Mgi Jnum | J:74346 |
| Mgi Id | MGI:2158117 | Doi | 10.1016/s0092-8674(01)00636-5 |
| Citation | Tahirov TH, et al. (2002) Mechanism of c-Myb-C/EBP beta cooperation from separated sites on a promoter. Cell 108(1):57-70 |
| abstractText | c-Myb, but not avian myeloblastosis virus (AMV) v-Myb, cooperates with C/EBP beta to regulate transcription of myeloid-specific genes. To assess the structural basis for that difference, we determined the crystal structures of complexes comprised of the c-Myb or AMV v-Myb DNA-binding domain (DBD), the C/EBP beta DBD, and a promoter DNA fragment. Within the c-Myb complex, a DNA-bound C/EBP beta interacts with R2 of c-Myb bound to a different DNA fragment; point mutations in v-Myb R2 eliminate such interaction within the v-Myb complex. GST pull-down assays, luciferase trans-activation assays, and atomic force microscopy confirmed that the interaction of c-Myb and C/EBP beta observed in crystal mimics their long range interaction on the promoter, which is accompanied by intervening DNA looping. |
