| First Author | Wegrowski Y | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 250 |
| Issue | 2 | Pages | 206-11 |
| PubMed ID | 9753608 | Mgi Jnum | J:50102 |
| Mgi Id | MGI:1289867 | Doi | 10.1006/bbrc.1998.9262 |
| Citation | Wegrowski Y, et al. (1998) Uridine diphosphoglucose dehydrogenase regulates proteoglycan expression: cDNA cloning and antisense study. Biochem Biophys Res Commun 250(2):206-11 |
| abstractText | Using a reverse-transcriptase-polymerase chain reaction approach human and murine UDPG-dehydrogenase (GDH) was cloned from fibroblast mRNAs. Human enzyme is 97% and 27% identical with its murine and E. coli orthologs. Murine mRNA of 3.1 kb size is expressed in all the tissue studied at a level independent of glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA. In human fibroblast in vitro, 2 GDH transcripts were observed. They were expressed proportionally to GAPDH. The simple pattern of human GDH Southern blotting suggests a single copy gene. An antisense oligonucleotide directed to the ATG region of the human enzyme inhibited 35S-sulphate incorporation into extracellular macromolecules, especially proteoglycans. These data indicate that GDH expression may regulate proteoglycan synthesis in the cells. |
