| First Author | Li Z | Year | 2012 |
| Journal | Cell Stem Cell | Volume | 11 |
| Issue | 5 | Pages | 663-75 |
| PubMed ID | 23122290 | Mgi Jnum | J:193721 |
| Mgi Id | MGI:5469253 | Doi | 10.1016/j.stem.2012.07.004 |
| Citation | Li Z, et al. (2012) Mouse embryonic head as a site for hematopoietic stem cell development. Cell Stem Cell 11(5):663-75 |
| abstractText | In the mouse embryo, the aorta-gonad-mesonephros (AGM) region is considered to be the sole location for intraembryonic emergence of hematopoietic stem cells (HSCs). Here we report that, in parallel to the AGM region, the E10.5-E11.5 mouse head harbors bona fide HSCs, as defined by long-term, high-level, multilineage reconstitution and self-renewal capacity in adult recipients, before HSCs enter the circulation. The presence of hemogenesis in the midgestation head is indicated by the appearance of intravascular cluster cells and the blood-forming capacity of a sorted endothelial cell population. In addition, lineage tracing via an inducible VE-cadherin-Cre transgene demonstrates the hemogenic capacity of head endothelium. Most importantly, a spatially restricted lineage labeling system reveals the physiological contribution of cerebrovascular endothelium to postnatal HSCs and multilineage hematopoiesis. We conclude that the mouse embryonic head is a previously unappreciated site for HSC emergence within the developing embryo. |
