Primary Identifier | MGI:3043744 | Allele Type | Targeted |
Attribute String | Hypomorph, Reporter | Gene | Gjb2 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A cassette containing IRES-lacZ and neo was inserted immediately downstream of the stop codon in exon 2. While undetectable by Northern blot analysis, transcript was identified by RT-PCR analysis indicating the amount of message in homozygous mutant mice to be severely reduced relative to that of wild-type. The inserted lacZ gene was expressed. |