| First Author | Lareyre JJ | Year | 1998 |
| Journal | Mol Reprod Dev | Volume | 50 |
| Issue | 4 | Pages | 387-95 |
| PubMed ID | 9669522 | Mgi Jnum | J:48464 |
| Mgi Id | MGI:1270029 | Doi | 10.1002/(SICI)1098-2795(199808)50:4<387::AID-MRD2>3.0.CO;2-E |
| Citation | Lareyre JJ, et al. (1998) Genomic organization and chromosomal localization of the murine epididymal retinoic acid-binding protein (mE-RABP) gene. Mol Reprod Dev 50(4):387-95 |
| abstractText | The murine epididymal retinoic acid-binding protein (mE- RABP) is specifically synthesized in the mouse mid/distal caput epididymidis and secreted in the lumen. In this report, we have demonstrated by Southern blot analysis of genomic DNA that mE-RABP is encoded by a single-copy gene. A mouse 129/SvJ genomic bacterial artificial chromosome (BAC) library was screened using a cDNA encoding the minor form of mE-RABP. One positive BAC clone was characterized and sequenced to determine the nucleo tide sequence of the entire mE-RABP gene. The molecular cloning of the mE-RABP gene completes the characterization of the 20.5-kDa- predicted preprotein leading to the minor and major forms of mE-RABP. Comparison of the DNA sequence of the promoter and coding regions with that of the rat epididymal secretory protein I (ESP I) gene showed that the mE-RABP gene is the orthologue of the ESP I gene that encodes a vat epididymal retinoic acid-binding protein. Several regulatory elements, including a putative androgen receptor binding site, ''CACCC-boxes'', NF-1, Oct-1, and SP-1 recognition sites, are conserved in the proximal promoter Analysis of the nucleotide sequence of the mE- RABP gene revealed the presence of seven exons and showed that the genomic organization is highly related to other genes encoding lipocalins. The mE-RABP gene was mapped by fluorescent in situ hybridization to the [A3-B] region of the murine chromosome 2. Our data, combined with that of others, suggest that the proximal segment of the mouse chromosome 2 may be a rich region for genes encoding lipocalins with a genomic organization highly related to the mE-RABP gene. Mol. Reprod. Dev. 50.387-395, 1998. (C) 1998 Wiley-Liss, Inc. |
