| First Author | Ivics Z | Year | 1997 |
| Journal | Cell | Volume | 91 |
| Issue | 4 | Pages | 501-10 |
| PubMed ID | 9390559 | Mgi Jnum | J:104042 |
| Mgi Id | MGI:3611077 | Doi | 10.1016/s0092-8674(00)80436-5 |
| Citation | Ivics Z, et al. (1997) Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell 91(4):501-10 |
| abstractText | Members of the Tc1/mariner superfamily of transposons isolated from fish appear to be transpositionally inactive due to the accumulation of mutations. Molecular phylogenetic data were used to construct a synthetic transposon, Sleeping Beauty, which could be identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase binds to the inverted repeats of salmonid transposons in a substrate-specific manner, and it mediates precise cut-and-paste transposition in fish as well as in mouse and human cells. Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis. |
