Primary Identifier | MGI:2675202 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Akt1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A cassette containing lacZ and neo was inserted into exon 1 at the start codon via homologous recombination. The insertion deleted the coding region of exon 1 which encodes 15 N-terminal residues. Endogenous protein was undetected by Western blot analysis of homozygous mutant heart tissue. |