| First Author | Willinger M | Year | 1981 |
| Journal | J Supramol Struct Cell Biochem | Volume | 17 |
| Issue | 1 | Pages | 79-86 |
| PubMed ID | 7321055 | Mgi Jnum | J:6671 |
| Mgi Id | MGI:55145 | Doi | 10.1002/jsscb.380170109 |
| Citation | Willinger M, et al. (1981) Neuronal differentiation in cultures of weaver (wv) mutant mouse cerebellum. J Supramol Struct Cell Biochem 17(1):79-86 |
| abstractText | In the present study we report for the first time a weaver (wv) gene dose effect on neuron survival and neurite formation in vitro. Dissociated cerebellar cells from postnatal 7- and 8-day-old normal (+/+), heterozygous weaver (+/wv) and homozygous weaver (wv/wv) mice were cultured as monolayers on poly-L-lysine coated glass. Cell death occurred rapidly in wv/wv cultures. Cell counts showed that less than 20% of the total neurons and neuronal precursors (identified by birthday radiolabeling techniques) survived by Day 3. Cell death was less extensive in +/wv cultures with 65% of the total neurons and 80% of the precursors surviving by Day 3. In contrast to wv/wv cultures, younger neurons survive better than the total population in +/wv cultures. The impairment of neurite formation over the first week is also proportional to the number of mutant genes as shown by quantitation of (a) the percentage of cells with neurites; (b) the percentage of cells with neurites of a given length class ith time; (c) the lengths of the longest process formed per cell. The mean longest neurite lengths obtained by computer digitization at 6 days in vitro were 41.8, 26.8, and 9.0 micron for +/+, +/wv, and wv/wv granule cells, respectively. |
