| First Author | Kirino Y | Year | 2007 |
| Journal | RNA | Volume | 13 |
| Issue | 9 | Pages | 1397-401 |
| PubMed ID | 17652135 | Mgi Jnum | J:170752 |
| Mgi Id | MGI:4947214 | Doi | 10.1261/rna.659307 |
| Citation | Kirino Y, et al. (2007) The mouse homolog of HEN1 is a potential methylase for Piwi-interacting RNAs. RNA 13(9):1397-401 |
| abstractText | Piwi-interacting RNAs (piRNAs) are a novel class of small regulatory RNAs that are expressed specifically and abundantly in germ cells. Mammalian piRNAs are 26-31 nucleotides in length and bind to Piwi proteins, but their function and biogenesis remain elusive. We previously showed that mammalian piRNAs are 2'-O-methylated at their 3' termini. The biosynthetic mechanism and function of this modification is unknown. Here, we report that the mouse homolog (mHEN1) of HEN1, a plant microRNA (miRNA) 2'-O-methyltransferase, is expressed specifically in testis and methylates 3' termini of piRNAs in vitro. These findings provide insight into the biogenesis of piRNAs. |
