| First Author | Kozak LP | Year | 1988 |
| Journal | J Biol Chem | Volume | 263 |
| Issue | 25 | Pages | 12274-7 |
| PubMed ID | 3410843 | Mgi Jnum | J:9318 |
| Mgi Id | MGI:57780 | Doi | 10.1016/s0021-9258(18)37751-2 |
| Citation | Kozak LP, et al. (1988) The mitochondrial uncoupling protein gene. Correlation of exon structure to transmembrane domains. J Biol Chem 263(25):12274-7 |
| abstractText | The mitochondrial uncoupling protein, a protein essential for the thermogenic properties of brown fat in mammals, is inserted in the inner mitochondrial membrane by means of six alpha-helical hydrophobic transmembrane domains. We have sequenced a complete cDNA and parts of the gene to determine that the mitochondrial uncoupling protein gene is composed of six exons, each of which encodes a transmembrane domain. We also show that transcription of the uncoupling protein gene is from a single start site; however, the use of alternative poly(A) addition signal sequences results in two mRNAs, the major species of 1221 nucleotides, not including the poly(A) tail, and a minor species of about 1600 nucleotides. The 5'-untranslated region of the mRNA is composed of 231 nucleotides, and the 3'-untranslated region contains 81 nucleotides prior to addition of the poly(A) tail. |
