| First Author | Patel H | Year | 2002 |
| Journal | Arch Biochem Biophys | Volume | 403 |
| Issue | 1 | Pages | 145-8 |
| PubMed ID | 12061812 | Mgi Jnum | J:76037 |
| Mgi Id | MGI:2183882 | Doi | 10.1016/S0003-9861(02)00203-5 |
| Citation | Patel H, et al. (2002) Mammalian mitochondrial methylenetetrahydrofolate dehydrogenase-cyclohydrolase derived from a trifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase. Arch Biochem Biophys 403(1):145-8 |
| abstractText | We have isolated the cDNA and the gene encoding the murine cytoplasmic methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (DCS). Comparison of these sequences with the 3'-untranslated region of the mitochondrial NAD(+)-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase (mt-DC) revealed areas of significant homology. Both exon and intron sequences of the synthetase domain of DCS are homologous to sequences in the untranslated region of mt-DC. A similar comparison between the mt-DC and the DCS sequences of humans as well as Drosophila supports the conclusion that in higher eukaryotes the bifunctional mt-DC replaced a trifunctional precursor through inactivation of the synthetase domain. The mt-DC should be considered in models of one-carbon folate fluxes in mammals. |
