| First Author | Chen M | Year | 2012 |
| Journal | Endocrinology | Volume | 153 |
| Issue | 9 | Pages | 4256-65 |
| PubMed ID | 22733970 | Mgi Jnum | J:189194 |
| Mgi Id | MGI:5444592 | Doi | 10.1210/en.2012-1113 |
| Citation | Chen M, et al. (2012) Gsalpha deficiency in the paraventricular nucleus of the hypothalamus partially contributes to obesity associated with Gsalpha mutations. Endocrinology 153(9):4256-65 |
| abstractText | The G protein alpha-subunit G(s)alpha mediates receptor-stimulated cAMP generation. Heterozygous inactivating G(s)alpha mutations on the maternal allele result in obesity primarily due to reduced energy expenditure in Albright hereditary osteodystrophy patients and in mice. We previously showed that mice with central nervous system (CNS)-specific G(s)alpha deletion on the maternal allele (mBrGs KO) also develop severe obesity with reduced energy expenditure and that G(s)alpha is primarily expressed from the maternal allele in the paraventricular nucleus (PVN) of the hypothalamus, an important site of energy balance regulation. We now generated mice with PVN-specific G(s)alpha deficiency by mating Single-minded 1-cre and G(s)alpha-floxed mice. Homozygous G(s)alpha deletion produced early lethality. Heterozygotes with maternal G(s)alpha deletion (mPVNGsKO) also developed obesity and had small reductions in energy expenditure. However, this effect was much milder than that found in mBrGsKO mice and was more prominent in males. We previously showed mBrGsKO mice to have significant reductions in melanocortin receptor agonist-stimulated energy expenditure and now show that mBrGsKO mice have impaired cold-induced brown adipose tissue stimulation. In contrast, these effects were absent in mPVNGsKO mice. mPVNGsKO mice also had minimal effects on glucose metabolism as compared with mBrGsKO mice. Consistent with the presence of G(s)alpha imprinting, paternal heterozygotes showed no changes in energy or glucose metabolism. These results indicate that although G(s)alpha deficiency in PVN partially contributes to the metabolic phenotype resulting from maternal G(s)alpha mutations, G(s)alpha imprinting in other CNS regions is also important in mediating the CNS effects of G(s)alpha mutations on energy and glucose metabolism. |
