| First Author | Shaknovich R | Year | 1992 |
| Journal | Mol Cell Biol | Volume | 12 |
| Issue | 11 | Pages | 5059-68 |
| PubMed ID | 1406681 | Mgi Jnum | J:2814 |
| Mgi Id | MGI:51335 | Doi | 10.1128/mcb.12.11.5059 |
| Citation | Shaknovich R, et al. (1992) Conformational activation of a basic helix-loop-helix protein (MyoD1) by the C-terminal region of murine HSP90 (HSP84). Mol Cell Biol 12(11):5059-68 |
| abstractText | A murine cardiac lambda gt11 expression library was screened with an amphipathic helix antibody, and a recombinant representing the C-terminal 194 residues of murine HSP90 (HSP84) was cloned. Both recombinant and native HSP90s were then found to rapidly convert a basic helix-loop-helix protein (MyoD1) from an inactive to an active conformation, as assayed by sequence-specific DNA binding. The conversion process involves a transient interaction between HSP90 and MyoD1 and does not result in the formation of a stable tertiary complex. Conversion does not require ATP and occurs stoichiometrically in a dose-dependent fashion. HSP90 is an abundant, ubiquitous, and highly conserved protein present in most eukaryotic cells. These results provide direct evidence that HSP90 can affect the conformational structure of a DNA-binding protein. |
