| Primary Identifier | MGI:6758655 | Allele Type | Transgenic |
| Attribute String | Inducible, Recombinase | Gene | Tg(Mnx1-cre/ERT2)#Rbro |
| Strain of Origin | C57BL/6 x CBA | Induced With | tamoxifen |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The bacterial artificial chromosome (BAC) clone RP24-351I23 containing the mouse Mnx1 (Hb9) gene was modified using recombineering bacterial strain to introduce the cre/ERT2-BGH polyA and Frt-Zeo-Frt selection cassette into the exon 1 replacing the Mnx1 coding region. The Frt-Zeo-Frt cassette is retained in the transgene but lacks a eukaryotic promoter. Three founder lines (4, 8, and 10) were generated. Line 10 gave low-level inducible activity and was terminated. Line 4 and Line 8 displayed similar inducible recombination activity in the desired cell populations and were maintained. Lines 4 and 8 were indistinguishable, and line 8 was used for the majority of studies described. The pound symbol (#) is used when no line is specified and/or lines are pooled. |
