| First Author | Mizuno Y | Year | 2008 |
| Journal | BMC Bioinformatics | Volume | 9 Suppl 12 |
| Pages | S16 | PubMed ID | 19091015 |
| Mgi Jnum | J:153381 | Mgi Id | MGI:4365325 |
| Doi | 10.1186/1471-2105-9-S12-S16 | Citation | Mizuno Y, et al. (2008) Predicted mouse peroxisome-targeted proteins and their actual subcellular locations. BMC Bioinformatics 9 Suppl 12:S16 |
| abstractText | BACKGROUND: The import of most intraperoxisomal proteins is mediated by peroxisome targeting signals at their C-termini (PTS1) or N-terminal regions (PTS2). Both signals have been integrated in subcellular location prediction programs. However their present performance, particularly of PTS2-targeting did not seem fitting for large-scale screening of sequences. RESULTS: We modified an earlier reported PTS1 screening method to identify PTS2-containing mouse candidates using a combination of computational and manual annotation. For rapid confirmation of five new PTS2- and two previously identified PTS1-containing candidates we developed the new cell line CHO-perRed which stably expresses the peroxisomal marker dsRed-PTS1. Using CHO-perRed we confirmed the peroxisomal localization of PTS1-targeted candidate Zadh2. Preliminary characterization of Zadh2 expression suggested non-PPARalpha mediated activation. Notably, none of the PTS2 candidates located to peroxisomes. CONCLUSION: In a few cases the PTS may oscillate from 'silent' to 'functional' depending on its surface accessibility indicating the potential for context-dependent conditional subcellular sorting. Overall, PTS2-targeting predictions are unlikely to improve without generation and integration of new experimental data from location proteomics, protein structures and quantitative Pex7 PTS2 peptide binding assays. |
