| First Author | Lichter K | Year | 2022 |
| Journal | Cell Rep | Volume | 40 |
| Issue | 12 | Pages | 111382 |
| PubMed ID | 36130490 | Mgi Jnum | J:329335 |
| Mgi Id | MGI:7343478 | Doi | 10.1016/j.celrep.2022.111382 |
| Citation | Lichter K, et al. (2022) Ultrastructural analysis of wild-type and RIM1alpha knockout active zones in a large cortical synapse. Cell Rep 40(12):111382 |
| abstractText | Rab3A-interacting molecule (RIM) is crucial for fast Ca(2+)-triggered synaptic vesicle (SV) release in presynaptic active zones (AZs). We investigated hippocampal giant mossy fiber bouton (MFB) AZ architecture in 3D using electron tomography of rapid cryo-immobilized acute brain slices in RIM1alpha(-/-) and wild-type mice. In RIM1alpha(-/-), AZs are larger with increased synaptic cleft widths and a 3-fold reduced number of tightly docked SVs (0-2 nm). The distance of tightly docked SVs to the AZ center is increased from 110 to 195 nm, and the width of their electron-dense material between outer SV membrane and AZ membrane is reduced. Furthermore, the SV pool in RIM1alpha(-/-) is more heterogeneous. Thus, RIM1alpha, besides its role in tight SV docking, is crucial for synaptic architecture and vesicle pool organization in MFBs. |
