| First Author | Gruenbaum-Cohen Y | Year | 2012 |
| Journal | Proc Natl Acad Sci U S A | Volume | 109 |
| Issue | 28 | Pages | 11211-6 |
| PubMed ID | 22736793 | Mgi Jnum | J:186396 |
| Mgi Id | MGI:5432271 | Doi | 10.1073/pnas.1116065109 |
| Citation | Gruenbaum-Cohen Y, et al. (2012) The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc Natl Acad Sci U S A 109(28):11211-6 |
| abstractText | A fundamental aspect of skeletal myogenesis involves extensive rounds of cell fusion, in which individual myoblasts are incorporated into growing muscle fibers. Here we demonstrate that N-WASp, a ubiquitous nucleation-promoting factor of branched microfilament arrays, is an essential contributor to skeletal muscle-cell fusion in developing mouse embryos. Analysis both in vivo and in primary satellite-cell cultures, shows that disruption of N-WASp function does not interfere with the program of skeletal myogenic differentiation, and does not affect myoblast motility, morphogenesis and attachment capacity. N-WASp-deficient myoblasts, however, fail to fuse. Furthermore, our analysis suggests that myoblast fusion requires N-WASp activity in both partners of a fusing myoblast pair. These findings reveal a specific role for N-WASp during mammalian myogenesis. WASp-family elements appear therefore to act as universal mediators of the myogenic cell-cell fusion mechanism underlying formation of functional muscle fibers, in both vertebrate and invertebrate species. |
