| First Author | Ciemerych MA | Year | 1999 |
| Journal | Zygote | Volume | 7 |
| Issue | 4 | Pages | 293-300 |
| PubMed ID | 10717947 | Mgi Jnum | J:60767 |
| Mgi Id | MGI:1353878 | Doi | 10.1017/s0967199499000696 |
| Citation | Ciemerych MA, et al. (1999) Control of duration of the first two mitoses in a mouse embryo. Zygote 7(4):293-300 |
| abstractText | The duration of M-phase is largely determined by the time necessary for the formation of a functional metaphase spindle and the correct alignment of all chromosomes on the metaphase plate. The spindle assembly checkpoint prevents the exit from M-phase before the proper alignment of all chromosomes on a metaphase plate in many cell types. In the present paper we show that the first mitotic M-phase of the mouse embryo lasts about 119 min, while the second embryonic M-phase lasts only about 70 min. Histone H1 kinase is activated rapidly during nuclear envelope breakdown in both mitoses. Its maximum, however, is followed by a plateau only during the first mitosis. In the second mitosis, the inactivation of histone H1 kinase activity follows its maximum directly. Histone H1 kinase is more stable in the cytoplasts obtained from mouse embryos during the first embryonic M-phase than during the second one. The stability of histone H1 kinase is greatly increased by the presence of the mitotic apparatus in both M-phases. The mitotic spindle assembly during the first and the second mitoses differs and the first metaphase spindle is stabilised during the period of maximum histone H1 kinase activity. These data show that an unknown developmentally regulated mechanism controls the duration of the two first mitoses in the mouse embryo. |
