Primary Identifier | MGI:6505285 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Ddx4 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | true | Is Wild Type | false |
description | The version of this allele that does not contain the neomycin selection cassette, Ddx4tm1.1Dcp, has been analyzed. |
molecularNote | The sequence encoding a cre-mOrange fusion protein was inserted into the Ddx4 (Mvh) locus by homologous recombination with a targeting vector in ES cells. Part of exon 1 and intron 1 were replaced by the cre-mOrange sequence. A PGK-neo selection marker also was inserted. The mOrange fluorescent reporter in the construct does not appear to be functional. |