| First Author | Hernandez-Hansen V | Year | 2004 |
| Journal | J Leukoc Biol | Volume | 75 |
| Issue | 1 | Pages | 143-51 |
| PubMed ID | 14525964 | Mgi Jnum | J:87475 |
| Mgi Id | MGI:2687173 | Doi | 10.1189/jlb.0503224 |
| Citation | Hernandez-Hansen V, et al. (2004) The Src kinase Lyn is a negative regulator of mast cell proliferation. J Leukoc Biol 75(1):143-51 |
| abstractText | Previous investigators have reported that deletion of the protein tyrosine kinase Lyn alters mast cell (MC) signaling responses but does not affect or reduces the cytokine-mediated proliferation of mouse bone marrow-derived MC (BMMC) precursors and of mature MC. We observed that Lyn-deficient mice have more peritoneal MC than wild-type (WT) mice. Studies to explore this unexpected result showed that Lyn(-/-) BM cells expand faster than WT cells in response to interleukin (IL)-3 and stem-cell factor over the 4-5 weeks required to produce a >95% pure population of granular, receptor with high affinity for immunoglobulin E-positive BMMC. Furthermore, differentiated Lyn(-/-) BMMC continue to proliferate more rapidly than WT BMMC and undergo less apoptosis in response to cytokine withdrawal. Additionally, Lyn(-/-) BMMC support greater IL-3-mediated phosphorylation of the prosurvival kinase, Akt, and the proliferative kinase, extracellular-regulated kinase 1/2. These results identify Lyn as a negative regulator of murine MC survival and proliferation. |
