| First Author | Lee CG | Year | 1998 |
| Journal | Genomics | Volume | 47 |
| Issue | 3 | Pages | 365-71 |
| PubMed ID | 9480750 | Mgi Jnum | J:46086 |
| Mgi Id | MGI:1197114 | Doi | 10.1006/geno.1997.5139 |
| Citation | Lee CG, et al. (1998) Molecular analysis of the cDNA and genomic DNA encoding mouse RNA helicase A. Genomics 47(3):365-71 |
| abstractText | RNA helicase A is an enzyme that possesses both RNA and DNA helicase activities. In this report, we describe the isolation of a mouse cDNA encoding RNA helicase A, The deduced amino acid sequence derived from mouse RNA helicase A cDNA exhibits 87 and 47% identity to its human and Drosophila homologs, respectively, Using Southern blot analysis employing a mouse backcross panel, we have assigned the mouse RNA helicase A gene to chromosome 1, mapping near the D1Bir20 locus at MGD position 67. Northern blot and primer extension analyses indicate that, although its level is variable, RNA helicase A appears to be expressed from a single transcription start site in all tissues tested. Sequence analysis of the upstream genomic DNA revealed that the promoter region lacks a TATA box and contains two high-affinity sites for Spl, one ISRE, a binding site for interferon regulatory factor, and three AP2-binding sites, These findings suggest that the transcriptional regulation of the RNA helicase A gene is complex. (C) 1998 Academic Press. |
