| First Author | Hession C | Year | 1992 |
| Journal | Biochem Biophys Res Commun | Volume | 183 |
| Issue | 1 | Pages | 163-9 |
| PubMed ID | 1371918 | Mgi Jnum | J:2315 |
| Mgi Id | MGI:50839 | Doi | 10.1016/0006-291x(92)91623-x |
| Citation | Hession C, et al. (1992) Cloning of murine and rat vascular cell adhesion molecule-1. Biochem Biophys Res Commun 183(1):163-9 |
| abstractText | Vascular cell adhesion molecule-1 (VCAM1) is a member of the immunoglobulin (Ig) superfamily which interacts with the integrin very late antigen 4 (VLA4). We have cloned the cDNAs for both murine and rat VCAM1 from endotoxin-treated lung libraries. Both sequences encode proteins with seven extracellular Ig-like domains, which show 75.9% and 76.9% identity, respectively, with human VCAM1. Both murine and human cell lines show VLA4-dependent binding to COS cells transiently expressing murine and rat VCAM1. Two mAbs, M-K/1 and M-K/2, which recognize an antigen on murine bone marrow stromal cell lines, bind to murine VCAM1 expressed in COS cells and block VCAM1-dependent adhesion, confirming that these mAbs recognize murine VCAM1. |
