| First Author | Araki M | Year | 1993 |
| Journal | Gene | Volume | 126 |
| Issue | 2 | Pages | 261-4 |
| PubMed ID | 7683304 | Mgi Jnum | J:12425 |
| Mgi Id | MGI:60672 | Doi | 10.1016/0378-1119(93)90377-f |
| Citation | Araki M, et al. (1993) Cloning and sequencing of mouse VCAM-1 cDNA. Gene 126(2):261-4 |
| abstractText | We report here the nucleotide sequence of a cDNA encoding the mouse vascular cell adhesion molecule-1 (VCAM-1), a member of the immunoglobulin gene superfamily. The 2972-bp cDNA encodes 739 amino acids (aa) and shows 76.4% and 75.9% similarity with the human VCAM-1 nucleotide (nt) and aa sequence, respectively. Three independent cDNA clones had a 7-Ig unit structure (molecule containing a seven 'immunoglobulin motif' repeat), and only sequences corresponding to the 7- (and not to the 6)-Ig unit VCAM were detected by reverse transcription polymerase chain reaction (RT-PCR), indicating that in the mouse this is the dominant, if not exclusive, form of this molecule. Unlike other adhesion molecules, the cytoplasmic tails (19 aa) of mouse and human VCAM-1 are identical, despite a 85.2% similarity only at the nt level, suggesting a critical functional role. |
