| First Author | Basler M | Year | 2006 |
| Journal | J Immunol | Volume | 176 |
| Issue | 11 | Pages | 6665-72 |
| PubMed ID | 16709825 | Mgi Jnum | J:125058 |
| Mgi Id | MGI:3723408 | Doi | 10.4049/jimmunol.176.11.6665 |
| Citation | Basler M, et al. (2006) An altered T cell repertoire in MECL-1-deficient mice. J Immunol 176(11):6665-72 |
| abstractText | Immunoproteasome subunits low-molecular mass polypeptide (LMP)2 and LMP7 affect Ag presentation by MHC class I molecules. In the present study, we investigated the function of the third immunosubunit LMP10/multicatalytic endopeptidase complex-like (MECL)-1 (beta2i) in MECL-1 gene-targeted mice. The number of CD8+ splenocytes in MECL-1-/- mice was 20% lower than in wild-type mice. Infection with lymphocytic choriomeningitis virus (LCMV) elicited a markedly reduced cytotoxic T cell (CTL) response to the LCMV epitopes GP276-286/Db and NP205-212/Kb in MECL-1-/- mice. The weak CTL response to GP276-286/Db was not due to an impaired generation of this epitope but was attributed to a decreased precursor frequency of GP276-286/Db-specific T cells. The expansion of TCR-Vbeta10+ T cells, which contain GP276-286/Db-specific cells, was reduced in LCMV-infected MECL-1-/- mice. Taken together, our data reveal an in vivo function of MECL-1 in codetermining the T cell repertoire for an antiviral CTL response. |
