| First Author | Borriello F | Year | 1994 |
| Journal | J Immunol | Volume | 153 |
| Issue | 11 | Pages | 5038-48 |
| PubMed ID | 7525722 | Mgi Jnum | J:21646 |
| Mgi Id | MGI:69577 | Doi | 10.4049/jimmunol.153.11.5038 |
| Citation | Borriello F, et al. (1994) Characterization of the murine B7-1 genomic locus reveals an additional exon encoding an alternative cytoplasmic domain and a chromosomal location of chromosome 16, band B5. J Immunol 153(11):5038-48 |
| abstractText | The murine B7-1 (mB7-1) molecule expressed on APCs delivers a costimulatory signal for T cell activation through its T cell counter-receptor CD28, resulting in T cell proliferation and IL-2 production. Signaling through the TCR in the absence of CD28 signaling results in T cell anergy. We have analyzed the genomic structure of mB7-1 and here describe the identification of a previously unrecognized sixth exon at the far 3' end of the locus, which encodes an alternative cytoplasmic domain. Reverse transcriptase-PCR amplification of mB7-1 transcripts demonstrates that exon 6 is functionally spliced to the transmembrane-encoding exon 4. Furthermore, using 5' rapid amplification of cDNA ends, we determined that the 5'-untranslated region extends over 1505 bp beyond the previously reported transcriptional start site. In addition, we report the chromosomal location of mB7-1 to chromosome 16, band B5. |
