| First Author | Mahadevaiah SK | Year | 2001 |
| Journal | Nat Genet | Volume | 27 |
| Issue | 3 | Pages | 271-6 |
| PubMed ID | 11242108 | Mgi Jnum | J:126641 |
| Mgi Id | MGI:3761772 | Doi | 10.1038/85830 |
| Citation | Mahadevaiah SK, et al. (2001) Recombinational DNA double-strand breaks in mice precede synapsis. Nat Genet 27(3):271-6 |
| abstractText | In Saccharomyces cerevisiae, meiotic recombination is initiated by Spo11-dependent double-strand breaks (DSBs), a process that precedes homologous synapsis. Here we use an antibody specific for a phosphorylated histone (gamma-H2AX, which marks the sites of DSBs) to investigate the timing, distribution and Spo11-dependence of meiotic DSBs in the mouse. We show that, as in yeast, recombination in the mouse is initiated by Spo11-dependent DSBs that form during leptotene. Loss of gamma-H2AX staining (which in irradiated somatic cells is temporally linked with DSB repair) is temporally and spatially correlated with synapsis, even when this synapsis is 'non-homologous'. |
